It depends on how long has it been since you got them. After the first month, my sister only took them out and cleaned them every morning but, put them right back in after. She is happy doing that but I take mine out twice a week to soak over night. During the other days, I use those Colgate mini brushes called Wisp. I am 58 and have had them since I was 25. My mother keeps hers in a cup and only puts them in if some one comes over or she goes out! She is 74 and has had them since she was 30. That is a great question better left to your dentist to answer and then do what feels best for you.