# Expanding Clollin's nano machine idea (wild thinking)

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• Updated 8 years ago
Just thinking about it Clollin's comments in the nano machine puzzle (just a bit in a slightly crazy manner)

I think probably the easiest? change to a molecule from an 'rna probe' is appending a single base at the end of a molecule.

so build your stable nano machine with a chain of 79 bases
and then re enter the same sequence on a design of 80 bases - and decide what the final extra base is going to be

and see if the shape can change significantly from appending the single base.

shape/no shape (a line) is probably the biggest change.

Anyway something to mess about with
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Posted 8 years ago

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If I understand your proposal correctly...you are suggesting to make the exact design with one less base - and stable! Then add the one base at the end and have the sequential shift of every base down to the one that creates the fold to also now be stable in the folded format. ???

the only way I can see that happening is if the loop that allows the movement was very close to the beginning so that not too many bases had to be shifted.

What do you think?

have you tried designing anything?
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imagine a sequence

GGGAAACCC that would clearly end up as a hairpin

can you create a sequence

GGGAAACCCX that is stable in a significantly different shape

at the moment that looks like it would be a hairpin - with a spare base on the end, unless the spare base was a C

but I was looking for a 'shape change' from maybe a line

so perhaps

GAAAAAAAAA might be the simplest example - it's a line unless you add a C

I guess that GAAAAAAAAAC takes up a lot more room than CGAAAAAAAAA

so adding C to the end of the chain ought to increase the volume of the GAAAAAAA by a bigger amount than just adding C would normally indicate

perhaps that has a mechanical - push effect, also it might change the density as a result. So that would seem to be a possible RNA 'machine'

but then I don't know quite where that would go - is it possible to extend that out to a much more complex shape?

Edward :)
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Had a further thought that perhaps this shape change (and thus density and volume change) affects internal cell pressures when RNA are synthesised in vitro ?
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Not sure I follow you on the change in density and volume. Can you try to make the example as a puzzle?
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