I'm working on a prototype web-based switch design tool. I need to program in the aptamer bonus in kcal. The current lab tool suggests it is 4.0 kcal independent of the aptamer. Some interactions with Nando years ago suggested it might be 4.86 kcal for FMN at least. The fact that expected maximal fold changes can vary by aptamer suggest to me that the actual bonus might vary for different aptamers. What is the real case? Is it 4.0kcal, 4.86, or does it really vary by aptamer, and if so, what values should I use?