when i was trying to solve problems, some questions came up.
We have repetition rule and limitation rule of adenine maximum 40%.
In repetition, i roughly know it could make some misfording. But i want to know the specific reason for why each type of NT sholuldnt have repetition.
In adenine limitation rule, they will conduct PCR to amplify their template DNA after getting some candidate. adenine is limited to maximum 40%. In DNA, it means G or C is more than 60%. So it means it could have many GC pairs. But i know that many GCpairs make PCR hard since they cause high temperature. So i think not upper bound but lower bound must be provided in Adeninde. How do you think?